Contact an Account ManagerHPLC of O-methyl-catecholamine metabolites as DANS derivatives
Summary
Type: HPLC
Application #: 101990
Catalog#: 720023.40 - MN HPLC Column, Nucleosil C18, 100A, 10um, 4x250mm
Detection: fluorescence
Conditions: A) eluent: ethyl acetate - cyclohexane (75 + 50, v/v),
flow rate 0.84ml/min,
pressure: 50bar,
temperature: 22°C;
B) eluent: acetonitrile -water (64 + 36, v/v),
flow rate 1.3ml/min,
pressure: 6
Authors: Schwedt, G. et al.
Source: Z. Anal. Chem. 285 (1977) 381–384
Details
Legend: Fig. A) Separation of DANS-O-methylcatecholamines by adsorption chromatography,
injection volume 25µl (250ng amine each).
Fig. B) Separation of DANS-O-methylcatecholamines by reversed phase
chrom